Flow Cytometry

Beckman Coulter MoFlo XDP Cell Sorter

The XDP is a high speed cell sorter with 2 lasers, 7 colors, and 4 way sorting capability. It is equipped with a 488nm solid state laser and a 635nm diode laser. The XDP uses complete digital processing and can sort a high yield of cells at a greater efficiency. A variety of collection devices may be used including: microscope slides, micro-tubes, 12 x 75ml test tubes, 15 or 50ml falcon tubes, as well as multi-well plates (6, 24, 96, 384, or 1536). The machine is also capable of cooling or warming the sort collection device while sorting. This instrument is operated by staff on a fee for service basis.

For more information about the XDP, see http://www.coulterflow.com/bciflow/homena.php .

Beckman Coulter MoFlo Legacy Cell Sorter

The MoFlo is a high speed cell sorter with three lasers, 9 colors, and two way sorting capability. It is equipped with a solid state 488nm laser, an I90 argon ion multiline UV laser, and an I70C spectrum tunable laser (providing wavelengths from 440nm to 568nm). A variety of collection devices may be used including: microscope slides, micro-tubes, 12 x 75ml test tubes, 15 or 50ml falcon tubes, as well as multi-well plates (6, 24, 96, or 384). This instrument is operated by staff on a fee for service basis.

For more information about the MoFlo, see http://www.coulterflow.com/bciflow/homena.php

BD FACS Aria Cell Sorter

The FACS Aria cell sorter is equipped with three lasers (488, 405, and 635nm), 12 colors, and 4 way sorting capability. It also contains a Photo Multiplier Tube (PMT) for Forward Scatter (FSC) for greater dynamic range among smaller particles. The Aria can sort into a variety of collection devices including: microscope slides, micro-tubes, 12 x 75ml test tubes, 15 or 50ml falcon tubes, as well as multi-well plates (6, 24, 96, or 384). The fixed nozzle eliminates the need for alignment with every run, and makes this instrument easier to use than the MoFlo.  For this reason, people who anticipate heavy use of the instrument can be trained to use it independently.  Users who needed it less frequently can have their samples run by the facility.

For more information see http://www.bdbiosciences.com/home/ .

BD LSRII

This analyzer has 4 lasers (405, 488, 561, and 633 nm) and can be used to analyze cells stained with many different fluorophores (fluorescent proteins, DsRed, mCherry, APC, and phycoerythrin, among others). Like the FACSARIA, the LSRII has a PMT option to allow a greater dynamic range for FSC. The high throughput sampler (HTS) allows cells to be taken from a 96 or 384 well plate. Researchers can be trained to use this instrument independently. For more information see http://www.bdbiosciences.com.