Liquid Handling

BioTek Elx405 Plate Washer

This programmable instrument is ideal for rapid aspiration and dispensing of liquids in microplates.  Applications include bulk reagent dispensing, particle washing, and other HTS protocols in microtiter wells. For more information, see http://www.biotek.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

Biotek Plate Washer Protocol (MS Word)

Tecan Genesis

This robot has eight independent pipetting tips for automated liquid handling in a variety of vessels including microtiter plates, tubes, and slides. Applications include:

• In situ hybridization of up to 48 slides using the Genepaint system
• PCR setup 
• Reagent dispensing
• Sample dilution and reconfiguration
• Serial dilutions

For more information, see http://www.tecan.com/.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

Beckman BioMek FX

This 96-channel pipetting instrument is used for high-throughput liquid-handling applications in microtiter plates. Applications include:

• Plasmid purifications
• PCR Purification
• PCR setup
• Plate-to-plate transfers or replications
• Compression or expansion of samples in 96- or 384-well plates.

For more information, see http://www.beckman.com.

Thermo MultiDrop

This dispensing device is used to rapidly fill the wells of 96-well or 384-well standard-height microtiter plates. The instrument uses a peristaltic pump to dispense in 5µl increments down to a minimum volume of 5 µl. For more information see http://www.thermo.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

Thermo MultiDrop Micro

This dispensing device is used to rapidly fill the wells of 96-well or 384-well standard-height microtiter plates. The instrument uses a peristaltic pump to dispense in 1µl increments down to a minimum volume of 1 µl. For more information see http://www.thermo.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

BioTek µFill

This dispensing device is used to rapidly fill the wells of 24, 96, or 384-well standard-height or deep-well microtiter plates. The instrument uses a syringe pump to dispense in 1µl increments down to a minimum volume of 5 µl. For more information see http://www.biotek.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

Biotek µFill Protocol (MS Word)

Staccato Sciclone Cell Station Assay

This Laboratory Information Management System (LIMS) is ideal for generating automated growth curve data for cells in microtiter plates. It has a capacity of 44 plates and the associated Victor3 Multilabel Plate Reader is capable of reading UV/Vis Absorbance, Fluorescence, and Luminescence.

For more information, see http://www.caliperls.com.

To learn about using this instrument, please contact Christian Daly (cdaly@cgr.harvard.edu, 617-496-7105).