Flow Cytometry

The Bauer Core has three flow cytometers on which we offer a combination of training and service. For more information about running a flow cytometry experiment and the training and services offered, please see Flow Cytometry Techniques & Protocols.

MoFlo Cell Sorter

The MoFlo is a high-speed cell sorter with three lasers: two I90 argon ion lasers (currently running 488 nm and multiline UV), and an I70 C spectrum laser (providing 647 and 568 nm wavelengths). This instrument is usually operated by facility staff on a fee-for-service basis. Frequent users may be trained to run their own samples on the MoFlo with assistance from the staff. For more information about the MoFlo, see http://www.dakocytomation.com.

To learn about using this instrument , please contact Brian Tilton (btilton@cgr.harvard.edu, 617-495-0676).

BD FACSAria

This sorter has 3 lasers (488, 633 and 405 nm) and can be used to sort cells stained with many different fluorophores, including fluorescent proteins (CFP, GFP, and YFP). Forward Scatter (FSC) has a PMT that increases its dynamic range and is able to detect particles as small as 0.2 microns. The instrument can sort into a variety of vessels, including 15ml tubes, microtubes, and microtiter plates which can be cooled or heated during sorting. The fixed nozzle eliminates the need for alignment with every run, and makes this instrument easier to use than the MoFlo. For this reason, people who anticipate heavy use of the instrument can be trained to use it independently. Users who needed it less frequently can have their samples run by the facility. For more information see http://www.bdbiosciences.com

To learn about using this instrument, please contact Brian Tilton (btilton@cgr.harvard.edu, 617-495-0676).

BD LSRII

This analyzer has 4 lasers (405, 488, 561, and 633 nm) and can be used to analyze cells stained with many different fluorophores (fluorescent proteins, DsRed, mCherry, APC, and phycoerythrin, among others). Like the FACSARIA, the LSRII has a PMT option to allow a greater dynamic range for FSC. The high throughput sampler (HTS) allows cells to be taken from a 96 well plate. Researchers can be trained to use this instrument independently. For more information see http://www.bdbiosciences.com.

To learn about using this instrument, please contact Brian Tilton (btilton@cgr.harvard.edu, 617-495-0676).