The Bauer Core has a number of instruments for creating, processing and analyzing microarrays. For more information about designing an experiment using either home-made or commercially available arrays please see Microarray Techniques & Protocols.

Affymetrix GeneChip Instrumentation

Affymetrix GeneChip Instrumentation

The hybridization oven can accommodate 64 arrays and the two fluidics stations can each process up to four arrays at a time. The model 3000 7G scanner has a solid-state green laser which scans at 0.51 µm pixelation with reduced photobleaching. For more information, see http://www.affymetrix.com.

To learn about using this instrument, please contact Jennifer Couget (jcouget@cgr.harvard.edu, 617-495-9402).
Agilent Scanner

Agilent Scanner

This laser-based scanner can be used for Agilent microarrays and homemade two-color (Cy3 and Cy5) microarrays. The carousel enables automated scanning of up to 48 slides. For more information, see http://www.chem.agilent.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

Agilent Scanner Protocol (MS Word)
Axon GenePix 4000B and 4000A Scanners

Axon GenePix 4000B Scanners

These confocal laser scanners are used to analyze standard two-color (Cy3 and Cy5) microarrays. GenePix software allows for adjustable scan area and resolution, as well as automated spot finding. The Genepix 4000B scans at resolutions between 5 and 100 microns. For more information, see http://www.moleculardevices.com.

To learn about using this instrument, please contact Christian Daly (cdaly@cgr.harvard.edu, 617-496-7105).

Axon GenePix Protocol (MS Word)
Genomic Solutions OmniGrid 100

Genomic Solutions OmniGrid 100

This high-throughput microarrayer has a capacity of 100 slides printed with up to 48 pins. For more information, see http://www.genomicsolutions.com.

To learn about using this instrument, please contact Christian Daly (cdaly@cgr.harvard.edu, 617-496-7105).

OmniGrid Protocol (MS Word)







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