Sample Analysis

Agilent Bioanalyzer 2100

The BioAnalyzer can be used for rapidly analyzing RNA, DNA, and proteins. This instrument uses lab-on-a-chip technology to perform automated quality control, quantification, and sizing of samples. Applications include:

• Microarray sample quality control
• PCR optimization
• Analysis of protein purifications

For more information, see http://www.chem.agilent.com.

To learn about using this instrument, please contact Jennifer Couget (jcouget@cgr.harvard.edu, 617-495-9402).

Agilent 8453 Spectrophotometer

This diode-array UV–visible spectrophotometer has a wavelength range of 190 to 1100 nm, and is used for sample analysis in cuvettes. For more information, see http://www.chem.agilent.com.

NanoDrop ND-1000 Spectrophotometer

The Nanodrop is a UV-Visible Spectrophotometer designed to read 1-2 µl samples without a cuvette. Most samples can be run without dilution. It has a wavelength range of 220-750nm. For more information see http://www.nanodrop.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

NanoDrop Protocol (MS Word)

Spectramax Plate Readers

The Spectramax Plus 384 is a UV–visible absorbance spectrophotometer used for sample analysis in microtiter wells or cuvettes. The instrument has a pathlength correction feature.

Plus 384 Protocol (MS Word)

The Spectramax Gemini XS is used for fluorescence measurements in microtiter plates.  It can be used for emission and excitation wavelengths between 250 and 850 nm.

Gemini Protocol (MS Word)

For more information, see http://www.moleculardevices.com.

Tecan Safire2

The Safire2 is multimode tunable plate reader capable of reading fluorescense and absorbance. Plates can be read multiple times with shaking between each read.

For more information, see http://www.tecan.com.