Sequencing: Illumina

 

Illumina (Solexa) Genome Analyzer

Illumina NextSeq 500

This system can configured to meet researchers' individual needs.

  • Reads up to 150 bp long
  • Single Read or Paired End
  • Two flow cell sizes:
    • High - up to 400 million clusters
    • Mid - up to 130 million clusters
  • Indexing/barcoding supported

For more information on the HiSeq, see our Illumina techniques page, or visit http://www.illumina.com.

Before beginnig a project, please contact Christian Daly (cdaly@cgr.harvard.edu) to discuss your experimental set-up. For pricing, please see the price list.

Illumina (Solexa) Genome Analyzer

Illumina HiSeq 2500

This system can can be configured to meet researchers' individual needs.

  • Reads up to 150 bp long
  • Single Read or Paired End
  • Two flow cell sizes:
    • "High Output" v4 - 8 lanes, up to 250 million clusters each
    • Rapid Run - 2 lanes, up to 150 million clusters each
  • Indexing/barcoding supported

For more information on the HiSeq, see our Illumina techniques page, or visit http://www.illumina.com.

Before beginnig a project, please contact Christian Daly (cdaly@cgr.harvard.edu) to discuss your experimental set-up. For pricing, please see the price list.

Illumina (Solexa) Genome Analyzer

Illumina HiSeq 2000

This system can generate up to 200 million clusters per sample and is usually used for short reads using standard "high output" v3 chemistry. Indexing/barcoding is supported.

For more information on the HiSeq, see our Illumina techniques page, or visit http://www.illumina.com.

Before beginnig a project, please contact Christian Daly (cdaly@cgr.harvard.edu) to discuss your experimental set-up. For pricing, please see the price list.

Illumina (Solexa) Genome Analyzer

IntegenX Apollo 324

This instrument automates next-generation sequencing library preparation using bead technology.

  • Reagent kits are available for DNA sequencing, RNA-Seq or ChIP-seq.
  • Compatible with indexing/multiplexing.
  • Prepare 1-48 samples at a time.

For more information on the Apollo 324, see our Illumina techniques page or visit http://integenx.com/products/apollo-324/.

To learn about using this instrument, please contact Jennifer Couget (jcouget@cgr.harvard.edu, 617-495-9402).

Ancillary Equipment for Sequencing Projects

The core offers instruments for sample shearing, size selection, library QC and quantification.

Illumina (Solexa) Genome Analyzer

Pippin Prep

This instrument automates nucleic acid size selection using convenient gel cassettes.

  • Choose broad or tight size range cuts.
  • Choose the % gel that will allow for selection of your desired size.
  • Prepare 4 samples at a time along with a reference.

For more information on the Pippin Prep, see our Illumina techniques page or visit http://www.sagescience.com/.

To learn about using this instrument, please contact Jennifer Couget (jcouget@cgr.harvard.edu, 617-495-9402)

 

 

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