Sequencing: Illumina

	Illumina HiSeq 2000 and 2500 These systems generate up to 200 million reads per sample and can be configured to meet researcher's individual needs. Reads from 50 to 150 bp long Single Read or Paired End Standard Run or Rapid Run Indexing/barcoding supported For more information on the HiSeq, see our Illumina techniques page, or visit http://www.illumina.com. Before beginnig a project, please contact Christian Daly (cdaly@cgr.harvard.edu) to discuss your experimental set-up. For pricing, please see the price list.
	Illumina MiSeq This smaller output system generates up to 15 million reads per sample. Libraries generated for the HiSeq systems will work on the MiSeq. Unique features/applications of the Miseq include: Reads up to 250 bp long Amplicon sequencing Library QC For more information on the MiSeq, see our Illumina techniques page, or visit http://www.illumina.com. Before beginnig a project, please contact Christian Daly (cdaly@cgr.harvard.edu) to discuss your experimental set-up. For pricing, please see the price list.
	IntegenX Apollo 324 This instrument automates next-generation sequencing library preparation using bead technology. Reagent kits are available for DNA sequencing, RNA-Seq or ChIP-seq. Compatible with indexing/multiplexing. Prepare 1-32 samples at a time. For more information on the Apollo 324, see our Illumina techniques page or visit http://integenx.com/products/apollo-324/. To learn about using this instrument, please contact Jennifer Couget (jcouget@cgr.harvard.edu, 617-495-9402).
Ancillary Equipment for Sequencing Projects The core offers instruments for sample shearing, size selection, library QC and quantification. Covaris Shearing Qubit and Bioanalyzer Real Time PCR
	Pippin Prep This instrument automates nucleic acid size selection using convenient gel casettes. Choose broad or tight size range cuts. Choose the % gel that will allow for selection of your desired size. Prepare 4 samples at a time along with a reference. For more information on the Pippin Prep, see our Illumina techniques page or visit http://www.sagescience.com/. To learn about using this instrument, please contact Jennifer Couget (jcouget@cgr.harvard.edu, 617-495-9402)