Thermal Cycling

MJ Opticon 2

This system is used for real-time quantitative PCR in 96-well plates. Features include a gradient capability for protocol optimization and a linear dynamic range of eight orders of magnitude. The system is capable of two-color detection:

• 523-543nm (FAM, SYBR)
• 540-700nm (HEX, TET, TAMRA, VIC)

For more information on this instrument, see http://www.bio-rad.com.

To learn about using this instrument, please contact Christian Daly cdaly@cgr.harvard.edu, 617-496-7105).

Stratagene MX3000p

This system is used for real-time quantitative PCR in 96-well plates. It has a linear dynamic range of seven orders of magnitude and is capable of four-color detection:

• FAM/SYBR Green I (492nm-516nm)
• HEX/JOE/VIC (535nm-555nm)
• ROX/Texas Red (585nm-610nm)
• Cy5 (635nm-665nm).

For more information on this instrument, see http://www.mx3000p.com.

To learn about using this instrument, please contact Christian Daly cdaly@cgr.harvard.edu, 617-496-7105).

Applied Biosystems 7900HT

This system is used for real-time quantitative PCR in 96-well plates and TaqMan Low Density Array cards. Features include a fast cycling mode and a linear dynamic range of nine orders of magnitude. The system is capable of detecting the following probes:

SYBRGreen I, FAM, VIC, JOE, NED, ROX, TAMRA, and TET

For more information on this instrument, see http://www.appliedbiosystems.com.

To learn about using this instrument, please contact Christian Daly cdaly@cgr.harvard.edu, 617-496-7105).

MJ Tetrads(4)

These thermal cyclers can be used for high-throughput PCR and cycle sequencing applications. Each instrument has a capacity of four 96-well or 384-well PCR plates.

For more information on this instrument, see http://www.bio-rad.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).