Homemade Microarrays
Commerical: Affymetrix
Commerical: Agilent
Alphabetical List of Microarray Protocols

Protocols and Reagents

The FAS Center uses the following protocols to prepare Affymetrix GeneChip® samples.

Tissue and RNA isolation
RNA quality
Fragmentation Protocol (MS Word)
Hybridization Protocol (MS Word)
Quality Control
Reagent List (PDF)

All Affymetrix procedures and protocols can be found in the Affymetrix GeneChip® Expression Analysis Technical Manual. This manual can be downloaded from the Affymetrix website at www.affymetrix.com after registration. Registration is free.