|
Task |
Time allocated |
1 |
Prepare positive and negative DNA controls |
Variable |
2 |
Optimize PCR |
Variable |
3 |
PCR all plates, run product on agarose
gels |
~ 10 plates/day |
4 |
Verify that fragment lengths match
expected lengths |
1-2 days |
5 |
Determine which genes, if any, need
to be reamplified and Re-PCR these genes. |
Variable |
6 |
Replace reamplified genes in original
96-well position |
Variable |
7 |
Purify PCR product and controls |
~ 20 plates/day |
8 |
Print controls (test print) |
~1 day |
9 |
Prepare polyA-RNA for test probe (to
hybridize to control slides) |
~2 days |
10 |
Block control slides |
1 hour |
11 |
Hybridize control slides with test
probe |
1-2 days |
12 |
Make .gal file for spot identification |
1-2 days |
13 |
Transfer purified DNA, including controls,
from 96-well plates into 384-well plates |
1day |
14 |
Print full arrays |
1-2 days |
