Illumina Sequencing

The Center supports massively parrallel high throughput sequencing using two Illumina Genome Analyzer II instruments. These sequencers generate millions of short reads per sample and enable a wide variety of applications including

DNA sequencing
Gene Expression
Small RNA
ChIP-Seq

Reads can be as short as 18 bp or can be over 75 bp, depending on the researchers needs. Optional "paired end" reads make sequence mapping easier. Multiple samples can be tagged and be combined in order to make the most of the system's output.

Researchers generate their own sample libraries using Illumina's kits and protocols and submit these libraries to the Center staff for sequencing according to pre-arranged conditions. Each flow cell contains 8 channels, enough for 7 samples and 1 control. All the channels on a flow cell must be used at the same time, but they may be shared by different groups. If you are interested in sharing a flow cell, please talk to us and we'll try to help match you with someone.

For more information, please contact Christian Daly (cdaly@cgr.harvard.edu) to discuss your experimental set-up. Registered users may submit a data analysis request using the Rearch Computing Group's submission page. For pricing, please see our price list.